Institut Pasteur (Paris, FR)
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Consequences of Cas9 cleavage in the chromosome of Escherichia coli
L Cui, D Bikard
Nucleic acids research 44 (9), 4243-4251, 2016
One-step cloning and chromosomal integration of DNA
F St-Pierre, L Cui, DG Priest, D Endy, IB Dodd, KE Shearwin
ACS synthetic biology 2 (9), 537-541, 2013
A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9
L Cui, A Vigouroux, F Rousset, H Varet, V Khanna, D Bikard
Nature communications 9 (1), 1912, 2018
Genome-wide CRISPR-dCas9 screens in E. coli identify essential genes and phage host factors
F Rousset, L Cui, E Siouve, C Becavin, F Depardieu, D Bikard
PLoS genetics 14 (11), e1007749, 2018
Tuning dCas9's ability to block transcription enables robust, noiseless knockdown of bacterial genes
ST Antoine Vigouroux, Enno Oldewurtel, Lun Cui, David Bikard
Molecular Systems Biology, 2018
Improving sequence-specific antimicrobials by blocking dna repair
D Bikard, CUI Lun, X Duportet, JF Rodriguez
Inhibition of NHEJ repair by type II-A CRISPR-Cas systems in bacteria
A Bernheim, A Calvo-Villamañán, C Basier, L Cui, EPC Rocha, ...
Nature communications 8 (1), 2094, 2017
Quantitation of the DNA tethering effect in long-range DNA looping in vivo and in vitro using the Lac and λ repressors
DG Priest, L Cui, S Kumar, DD Dunlap, IB Dodd, KE Shearwin
Proceedings of the National Academy of Sciences 111 (1), 349-354, 2014
On-target activity predictions enable improved CRISPR–dCas9 screens in bacteria
A Calvo-Villamañán, JW Ng, R Planel, H Ménager, A Chen, L Cui, ...
Nucleic acids research 48 (11), e64-e64, 2020
Enhancer-like long-range transcriptional activation by λ CI-mediated DNA looping
L Cui, I Murchland, KE Shearwin, IB Dodd
Proceedings of the National Academy of Sciences 110 (8), 2922-2927, 2013
Cas9 off-target binding to the promoter of bacterial genes leads to silencing and toxicity
W Rostain, T Grebert, D Vyhovskyi, PT Pizarro, ...
Nucleic Acids Research 51 (7), 3485-3496, 2023
Within-patient evolution of plasmid-mediated antimicrobial resistance
J DelaFuente, L Toribio-Celestino, A Santos-Lopez, R León-Sampedro, ...
Nature ecology & evolution 6 (12), 1980-1991, 2022
A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9. Nat Commun 9: 1912
L Cui, A Vigouroux, F Rousset, H Varet, V Khanna, D Bikard
Lipoprotein DolP supports proper folding of BamA in the bacterial outer membrane promoting fitness upon envelope stress
D Ranava, Y Yang, L Orenday-Tapia, F Rousset, C Turlan, V Morales, ...
Elife 10, e67817, 2021
Clonetegration using OSIP plasmids: One-step DNA assembly and site-specific genomic integration in bacteria
L Cui, KE Shearwin
Synthetic DNA: Methods and Protocols, 139-155, 2017
Bacteriophage lambda repressor mediates the formation of a complex enhancer-like structure
L Cui, I Murchland, IB Dodd, KE Shearwin
Transcription 4 (5), 201-205, 2013
Genome-wide CRISPR-Cas9 screen in E. coli identifies design rules for efficient targeting
B Gutierrez, JW Ng, L Cui, C Becavin, D Bikard
bioRxiv, 308148, 2018
Repurposing site-specific recombinases for synthetic biology
L Cui, F St-Pierre, K Shearwin
Future Microbiology 8 (11), 1361-1364, 2013
Recombinant T7 RNA polymerase production using ClearColi BL21(DE3) and animal-free media for in vitro transcription
Q Liang, B Tu, L Cui
Applied Microbiology and Biotechnology 108 (1), 1-15, 2024
Outer membrane lipoprotein DolP interacts with the BAM complex and promotes fitness during envelope stress response
D Ranava, Y Yang, L Orenday-Tapia, F Rousset, C Turlan, L Cui, ...
bioRxiv, 2020.09. 23.308296, 2020
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